NIST · Open Source · NIST AI
Bundled Lentiviral Vector Copy Number Measurements
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★ Tier-1 Source
NIST invites participants to an Interlaboratory Testing Program to evaluate the suitability and utility of the genomic DNA test materials to serve as reference materials or controls for the measurements of the integrated lentiviral vector copy number (VCN) into the genome of target cells.
Key facts
- DNA prepared from the cell lines was suspended in 1X TE buffer (10 mM TRIS 1 mM EDTA pH 8.0) at a concentration of approximately 25 ng/µL (nominal concentration was measured) and stored at 4 ⁰C
- The test set will consist of 5 vials containing genomic DNA extracted from the VCN cell lines, each vial will contain 80 µL of genomic DNA at a total of approximately 2 µg DNA
- The transduced Jurkat cells were clonally isolated to produce stable cell lines with N=1, N=2, N=3, and N=4 copies of the integrated provirus
- Reference standards for accurate validation and optimization of assays that determine integrated lentiviral vector copy number in transduced cells”, Scientific Reports (2021) 11, article Number 389
Summary
NIST will provide the DNA samples free of charge to the participants for their evaluation using their own measurement methods. The laboratories will measure VCN using the measurement techniques of their choice and NIST will coordinate the study and provide data analysis of the data provided by the participants. NIST has produced genomic DNA test materials from transduced Jurkat cell lines with integrated lentiviral vector copy number (VCN) for evaluation as a candidate reference material.